An additional applying of all of our UTF1 reporter cassette ( 31), we working target vector pattP4X-PGKssPuro-UTF1-EGFP Here, the latest UTF1 promoter/enhancer points handle EGFP phrase, since the puromycin opposition cassette is actually constitutively conveyed just after targeted genomic integration (Contour 4A). Co-transfection of the address vector pattP4X-PGKssPuro-UTF1-EGFP and Int-C3NLS expression vector accompanied by solutions […]